HIST1H3A anticorps (AA 131-230) (Cy3)

N° du produit ABIN759509

L’anticorps Lapin Polyclonal anti-HIST1H3A a été validé pour WB, FACS, IF (cc) et IF (p). Il convient pour détecter HIST1H3A dans des échantillons de Humain, Souris, Rat et Mouton.

Aperçu rapide pour HIST1H3A anticorps (AA 131-230) (Cy3) (ABIN759509)

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivité: Humain, Souris, Rat, Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIST1H3A est conjugé à/à la Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Détail du produit anti-HIST1H3A anticorps (Cy3)

Épitope: AA 131-230

Réactivité croisée: Humain, Souris, Rat, Mouton

Homologie: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human HIF3 alpha

Isotype: IgG

Information d'application

Indications d'application: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur HIST1H3A

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Autre désignation: H3 alpha

Sujet:

Synonyms: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF3-alpha-1, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A

Background: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity).

ID gène: 64344

UniProt: Q9Y2N7